Given the mRNA sequence AUG ACU AGC UGG GGG UAU UAC UUU UAG, fill in the missing DNA sequence, the tRNA anticodons, and the amino acid sequence.

What you are being asked to complete

You are given the mRNA, so you can (1) translate it into amino acids by reading codons (3 bases at a time), (2) write the matching tRNA anticodons by base pairing, and (3) write a matching DNA sequence by replacing $U$ with $T$ (coding strand) or by taking the complement (template strand).

Split the mRNA into codons

mRNA (5'→3'):

AUG | ACU | AGC | UGG | GGG | UAU | UAC | UUU | UAG

Translate codons to amino acids

Using the genetic code:

• $AUG$ = Met (start)
• $ACU$ = Thr
• $AGC$ = Ser
• $UGG$ = Trp
• $GGG$ = Gly
• $UAU$ = Tyr
• $UAC$ = Tyr
• $UUU$ = Phe
• $UAG$ = Stop

So the amino acid sequence is: Met–Thr–Ser–Trp–Gly–Tyr–Tyr–Phe–Stop

Find the tRNA anticodons

tRNA anticodons base-pair with mRNA codons ($A$ with $U$, $C$ with $G$) and are often written 3'→5':

• $AUG$ → $UAC$
• $ACU$ → $UGA$
• $AGC$ → $UCG$
• $UGG$ → $ACC$
• $GGG$ → $CCC$
• $UAU$ → $AUA$
• $UAC$ → $AUG$
• $UUU$ → $AAA$
• $UAG$ → no tRNA anticodon (stop codon is recognized by a release factor)

Write one correct DNA sequence

If you want the DNA coding (sense) strand, it matches the mRNA except $T$ replaces $U$:

DNA coding (5'→3'): ATG ACT AGC TGG GGG TAT TAC TTT TAG

(For reference, the DNA template (antisense) strand that is read by RNA polymerase would be complementary: 3'→5' TAC TGA TCG ACC CCC ATA ATG AAA ATC.)